rabbit polyclonal anti tgm2 antibody  (Proteintech)

Journal: BMC oral health

Article Title: Role of transglutaminase 2 in promoting biglycan synthesis in idiopathic gingival fibromatosis.

doi: 10.1186/s12903-024-05211-8

Figure Lengend Snippet: Fig. 2 (a) Gene Ontology enrichment analysis of the differentially expressed genes; cellular component. (b) Volcano map of differentially expressed genes in the human gingival fibroblasts (hGFs) of patients with idiopathic gingival fibromatosis (IGF)1/2 and periodontal disease (PD). Arrows indicate TGM2. (c) mRNA expression of TGM2 in the hGFs of a patient with PD and IGF. The bar graph shows the mean mRNA expression and standard deviation (SD), **p < 0.01, n = 3. (d) Protein levels of TGM2. The bar graph shows the protein level and standard deviation (SD), **p < 0.01, n = 3. (e) Immunofluores cence of hGFs isolated from a patient with PD and IGF1. Green, TGM2; red; actin; blue, nuclear. The bar indicates 75 mm

Article Snippet: The primary antibody, rabbit anti-human biglycan (BGN) polyclonal antibody (16409- 1-AP, ProteinTech), rabbit anti-human TGM2 polyclonal antibody (ProteinTech), and goat anti-human type 1 collagen (1310-1, SouthernBiotech, AL, USA) were reacted at 4°C for 24 h. After the reaction was completed and the sections were washed with PBS, goat anti-rabbit IgG H&L (Alexa Fluor 488; Abcam) and chicken anti-goat IgG (H&L) Alexa Fluor 594 (A-21468; Thermo Fisher Scientific) were used as secondary antibodies.

Techniques: Expressing, Standard Deviation, Isolation

Journal: BMC oral health

Article Title: Role of transglutaminase 2 in promoting biglycan synthesis in idiopathic gingival fibromatosis.

doi: 10.1186/s12903-024-05211-8

Figure Lengend Snippet: Fig. 3 Expression of mRNA related to the extracellular matrix, COL1A1 (a), ACTA2 (b), and FN1 (c). The bar graph shows the mean level of mRNA expres sion and standard deviation (SD), n = 3. To investigate the mRNA expression related to the extracellular matrix stimulated with transforming growth factor (TGF)-β (10 ng/mL, 24 h). (d) TGM2. (e) COL1A1. (f) ACTA2. (g) FN1. The bar graph shows the mean mRNA expression and standard deviation (SD), *p < 0.05, **p < 0.01, n = 3

Article Snippet: The primary antibody, rabbit anti-human biglycan (BGN) polyclonal antibody (16409- 1-AP, ProteinTech), rabbit anti-human TGM2 polyclonal antibody (ProteinTech), and goat anti-human type 1 collagen (1310-1, SouthernBiotech, AL, USA) were reacted at 4°C for 24 h. After the reaction was completed and the sections were washed with PBS, goat anti-rabbit IgG H&L (Alexa Fluor 488; Abcam) and chicken anti-goat IgG (H&L) Alexa Fluor 594 (A-21468; Thermo Fisher Scientific) were used as secondary antibodies.

Techniques: Expressing, Standard Deviation

Journal: BMC oral health

Article Title: Role of transglutaminase 2 in promoting biglycan synthesis in idiopathic gingival fibromatosis.

doi: 10.1186/s12903-024-05211-8

Figure Lengend Snippet: Fig. 4 (a) The mRNA expression of POSTN and biglycan (BGN) related to the extracellular matrix stimulated with transforming growth factor (TGF)-β (10 ng/mL, 24 h). The mean mRNA expression and standard deviation (SD) are shown, *p < 0.05, **p < 0.01, n = 3. (b) Immunofluorescence images of gingival tissue in patients with drug-induced gingival enlargement (DIGE) and idiopathic gingival fibromatosis (IGF). BGN (green), COL1A1 (red), and nuclear (blue). The bar indicates 150 μm. (c) The effect of rhTGM2 on BGN mRNA expression in human gingival fibroblasts (hGF) isolated from a patient with IGF. TGM2 at the indicated dose (ng/mL) was introduced for 24 h. The mean mRNA expression and SD are shown, *p < 0.05, n = 3. (d) Protein levels of SP1 in hGFs isolated from a patient with periodontal disease (PD) and patients with IGF. The mean protein level and SD are shown, **p < 0.01, n = 3. (d) The effect of plicamycin on BGN mRNA expression. Plicamycin was applied at the indicated concentration (mM) for 24 h. The mean mRNA expression and SD are shown, **p < 0.01, n = 3. (f) Schema of the mechanisms underlying IGF development, showing that biglycan upregulation via SP1 causes TGM2 downregulation in gingival fibroblasts in IGF

Article Snippet: The primary antibody, rabbit anti-human biglycan (BGN) polyclonal antibody (16409- 1-AP, ProteinTech), rabbit anti-human TGM2 polyclonal antibody (ProteinTech), and goat anti-human type 1 collagen (1310-1, SouthernBiotech, AL, USA) were reacted at 4°C for 24 h. After the reaction was completed and the sections were washed with PBS, goat anti-rabbit IgG H&L (Alexa Fluor 488; Abcam) and chicken anti-goat IgG (H&L) Alexa Fluor 594 (A-21468; Thermo Fisher Scientific) were used as secondary antibodies.

Techniques: Expressing, Standard Deviation, Immunofluorescence, Isolation, Concentration Assay

Journal: Frontiers in Veterinary Science

Article Title: Canine polarized macrophages express distinct functional and transcriptomic profiles

doi: 10.3389/fvets.2022.988981

Figure Lengend Snippet: Identification of canine macrophage polarization by flow cytometry. Monocyte-derived macrophages were generated from blood of healthy dogs by in vitro culture with or without cytokines as described in methods, and expression of molecules associated with M1 and M2 macrophages was assessed using flow cytometry and cross-reactive antibodies iNOS, inducible Nitric Oxide Synthase; Arginase, TGM2, Transglutaminase 2; CD206, macrophage mannose receptor. (A) Primary antibody staining depicted in blue histograms, while isotype matched, irrelevant antibody staining depicted by red histograms. Marker abbreviation: M0, unstimulated macrophages; M1, macrophages activated with IFN-γ; M2, macrophages activated with IL-4 and IL-13; (B) CD86 expression. % positive cells of total cells immunostained (C) MFI, mean fluorescence intensity. Normalized to isotype controls.

Article Snippet: Primary antibodies used in the study included the following: rabbit polyclonal anti-human suppressor of cytokine signaling 1 (SOCS-1) (Bio-Rad, Hercules CA), rabbit polyclonal anti-mouse iNOS (#PA3-030A, Thermo Fisher, Waltham MA), mouse monoclonal anti-human arginase I (Arg1, clone 19) (BD Biosciences, San Jose, CA), rabbit polyclonal anti- mouse tissue transglutaminase 2 (TGM2) (Bio-Rad), mouse anti-human CD206-PE (macrophage mannose receptor) (Clone 3.29B1.10) (Beckman Coulter, Brea CA), rabbit polyclonal anti-mouse resistin-like molecule α (RELMα) (PeproTech, Rocky Hill NJ), sheep polyclonal anti-human indoleamine-pyrrole 2,3 dioxygenase (IDO) (Bio-Rad, Hercules, CA) and rabbit polyclonal CXCL10 (IP-10) (BioRad).

Techniques: Flow Cytometry, Derivative Assay, Generated, In Vitro, Expressing, Staining, Marker, Fluorescence

Journal: Frontiers in Veterinary Science

Article Title: Canine polarized macrophages express distinct functional and transcriptomic profiles

doi: 10.3389/fvets.2022.988981

Figure Lengend Snippet: Macrophage responses to polarization assessed by immunofluorescence staining for intracellular TGM2, arginase, or SOCS1 expression. (A–C) Immunofluorescence staining for TGM2 expression (cyan) by M0, M1, and M2 differentiated macrophages (left, middle, and right columns, respectively. (D) Mean fluorescence intensity (MFI) for intracellular TGM2 expression depicted in bar graphs. (E–G) Immunofluorescence staining for intracellular expression of arginase (red). MFI for arginase expression depicted in bar graph (H) . (I–K) Immunofluorescence staining for intracellular expression of SOCS1 (cyan). MFI for SOCS1 expression depicted in bar graph. (L) Values are plotted as mean ± SD. Statistical differences assessed using One-way ANOVA, followed by Tukey's multiple means adjustment (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001). Scale bar indicates 50 μm.

Article Snippet: Primary antibodies used in the study included the following: rabbit polyclonal anti-human suppressor of cytokine signaling 1 (SOCS-1) (Bio-Rad, Hercules CA), rabbit polyclonal anti-mouse iNOS (#PA3-030A, Thermo Fisher, Waltham MA), mouse monoclonal anti-human arginase I (Arg1, clone 19) (BD Biosciences, San Jose, CA), rabbit polyclonal anti- mouse tissue transglutaminase 2 (TGM2) (Bio-Rad), mouse anti-human CD206-PE (macrophage mannose receptor) (Clone 3.29B1.10) (Beckman Coulter, Brea CA), rabbit polyclonal anti-mouse resistin-like molecule α (RELMα) (PeproTech, Rocky Hill NJ), sheep polyclonal anti-human indoleamine-pyrrole 2,3 dioxygenase (IDO) (Bio-Rad, Hercules, CA) and rabbit polyclonal CXCL10 (IP-10) (BioRad).

Techniques: Immunofluorescence, Staining, Expressing, Fluorescence